Review



stealth sirna negative control (scramble)  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Thermo Fisher stealth sirna negative control (scramble)
    RT-qPCR validation of changes in expression of a subset of genes following BLID knockdown. RT-qPCR analysis of several genes in (A) MCF-7 and (B) MDA-MB-231 cells. β-Actin served as the internal control. (A) The y-axis title of the middle graph is identical to the y-axis title of the left graph. (B) The y-axis title of the middle-left graph is identical to the y-axis title of the far-left graph. *P<0.05 and **P<0.01 (BLID <t>shRNA</t> vs. the corresponding Scr shRNA group), n=3. shRNA, <t>short</t> <t>hairpin</t> <t>RNA;</t> <t>Scr,</t> <t>scramble</t> control; Ctl, control; RT-qPCR, reverse transcription-quantitative PCR; BLID, BH-3 like motif containing inducer of cell death.
    Stealth Sirna Negative Control (Scramble), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stealth+sirna+negative+control+(scramble)/stealth+rnai+sirna/pmc12217432-124-2-26
    Average 90 stars, based on 1 article reviews
    stealth sirna negative control (scramble) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "BLID is a drug-responsive target of FOXO3a and multi-omics analysis reveals survival mechanisms and therapeutic vulnerabilities in BLID-deficient breast cancer cells"

    Article Title: BLID is a drug-responsive target of FOXO3a and multi-omics analysis reveals survival mechanisms and therapeutic vulnerabilities in BLID-deficient breast cancer cells

    Journal: Oncology Letters

    doi: 10.3892/ol.2025.15155

    RT-qPCR validation of changes in expression of a subset of genes following BLID knockdown. RT-qPCR analysis of several genes in (A) MCF-7 and (B) MDA-MB-231 cells. β-Actin served as the internal control. (A) The y-axis title of the middle graph is identical to the y-axis title of the left graph. (B) The y-axis title of the middle-left graph is identical to the y-axis title of the far-left graph. *P<0.05 and **P<0.01 (BLID shRNA vs. the corresponding Scr shRNA group), n=3. shRNA, short hairpin RNA; Scr, scramble control; Ctl, control; RT-qPCR, reverse transcription-quantitative PCR; BLID, BH-3 like motif containing inducer of cell death.
    Figure Legend Snippet: RT-qPCR validation of changes in expression of a subset of genes following BLID knockdown. RT-qPCR analysis of several genes in (A) MCF-7 and (B) MDA-MB-231 cells. β-Actin served as the internal control. (A) The y-axis title of the middle graph is identical to the y-axis title of the left graph. (B) The y-axis title of the middle-left graph is identical to the y-axis title of the far-left graph. *P<0.05 and **P<0.01 (BLID shRNA vs. the corresponding Scr shRNA group), n=3. shRNA, short hairpin RNA; Scr, scramble control; Ctl, control; RT-qPCR, reverse transcription-quantitative PCR; BLID, BH-3 like motif containing inducer of cell death.

    Techniques Used: Quantitative RT-PCR, Biomarker Discovery, Expressing, Knockdown, Control, shRNA, Reverse Transcription, Real-time Polymerase Chain Reaction

    Related Articles

    other:

    Article Title: Intranasal CRMP2-Ubc9 Inhibitor Regulates Na V 1.7 to Alleviate Trigeminal Neuropathic Pain
    Article Snippet: For the corresponding set of experiments, trigeminal ganglion neurons (TGs) were transfected with EGFP and Invitrogen Stealth RNAiTM siRNA Negative Control (scramble siRNA) (cat. no. 12935300, Thermo Fisher Scientific) or CRMP2 siRNA (5′ GTAAACTCCTTCCTCGTGT-3′; obtained from Thermo Fisher Scientific) using the 4D-Nucleofector (P3 Primary Cell Solution, program DR 114; Lonza Biosciences).

    Article Title: BLID is a drug-responsive target of FOXO3a and multi-omics analysis reveals survival mechanisms and therapeutic vulnerabilities in BLID-deficient breast cancer cells
    Article Snippet: In addition, Stealth siRNA negative control (scramble) with the same supplier's proprietary sequence designed to minimize sequence homology to any known vertebrate transcript (cat. no. 12935300, Thermo Fisher Scientific, Inc; Invitrogen) was used.

    Article Title: Myosin Va interacts with the exosomal protein spermine synthase
    Article Snippet: Supplementary method Western blotting MCF-7 cells were plated and transfected in 6-well plates using DharmaFECT 1 Transfection Reagent (GE Healthcare) according to the manufacturer’s instructions, with the Stealth RNAiTM siRNA targeting MYO5A and the scramble sequence Stealth RNAiTM siRNA Negative Control High GC Duplex (Invitrogen), as previously described.

    Small Interfering RNA:

    Article Title: PGC-1α and PGC-1β increase CrT expression and creatine uptake in myotubes via ERRα.
    Article Snippet: .. Myotubeswere transfectedwith 60 pmol pre-designed Stealth RNAi TM short interfering RNA (siRNA) for the CrT (Slc6A8) (cat no. RSS328932 and RSS328934) or the Stealth RNAi siRNA Negative Control Medium GC (scramble siRNA; Invitrogen). .. Transfections were performed using 2 μL/well Lipofectamine 2000 Transfection Reagent diluted in Opti-MEM Reduced Serum Medium (Invitrogen) on day 5 of differentiation.

    Article Title: The Role of MKP-5 in Adipocyte-Macrophage Interactions during Obesity
    Article Snippet: Human 293A cells from the ATCC were cultured using DMEM containing 10% FBS and penicillin/streptomycin. .. RNA Interference Small interfering RNA (siRNA) oligoribonucleotides targeting MKP-5 (siMKP5; Stealth RNAi TM siRNA, Cat. ‣8927232) and nonsense scramble siRNA (siSC; Stealth RNAi TM siRNA Negative Control, Cat. ‣12935300) were synthesized by Thermo Fisher Scientific. ..

    Article Title: The protective role of the MKP-5-JNK/P38 pathway in glucolipotoxicity-induced islet β-cell dysfunction and apoptosis.
    Article Snippet: Hyperglycemia and hyperlipidemia (glycolipotoxicity)-triggered islet β-cell dysfunction is known to drive the progression of obesity-related type 2 diabetes, however the underlying mechanisms have not been clearly elucidated.. The current study aimed to investigate the role of mitogen-activated protein kinase phosphatase 5 (MKP-5) in islet cells under glucolipotoxic conditions.. Using gene overexpression and knockdown approaches, we demonstrated that MKP-5 could alleviate glucolipotoxicity-induced apoptosis via the endoplasmic reticulum (ER) stress and mitochondrial apoptosis pathways owing to the altered regulation of caspase family members and ER stress-related molecules in MIN6 and primary islet cells.

    Negative Control:

    Article Title: PGC-1α and PGC-1β increase CrT expression and creatine uptake in myotubes via ERRα.
    Article Snippet: .. Myotubeswere transfectedwith 60 pmol pre-designed Stealth RNAi TM short interfering RNA (siRNA) for the CrT (Slc6A8) (cat no. RSS328932 and RSS328934) or the Stealth RNAi siRNA Negative Control Medium GC (scramble siRNA; Invitrogen). .. Transfections were performed using 2 μL/well Lipofectamine 2000 Transfection Reagent diluted in Opti-MEM Reduced Serum Medium (Invitrogen) on day 5 of differentiation.

    Article Title: The Role of MKP-5 in Adipocyte-Macrophage Interactions during Obesity
    Article Snippet: Human 293A cells from the ATCC were cultured using DMEM containing 10% FBS and penicillin/streptomycin. .. RNA Interference Small interfering RNA (siRNA) oligoribonucleotides targeting MKP-5 (siMKP5; Stealth RNAi TM siRNA, Cat. ‣8927232) and nonsense scramble siRNA (siSC; Stealth RNAi TM siRNA Negative Control, Cat. ‣12935300) were synthesized by Thermo Fisher Scientific. ..

    Article Title: The protective role of the MKP-5-JNK/P38 pathway in glucolipotoxicity-induced islet β-cell dysfunction and apoptosis.
    Article Snippet: Hyperglycemia and hyperlipidemia (glycolipotoxicity)-triggered islet β-cell dysfunction is known to drive the progression of obesity-related type 2 diabetes, however the underlying mechanisms have not been clearly elucidated.. The current study aimed to investigate the role of mitogen-activated protein kinase phosphatase 5 (MKP-5) in islet cells under glucolipotoxic conditions.. Using gene overexpression and knockdown approaches, we demonstrated that MKP-5 could alleviate glucolipotoxicity-induced apoptosis via the endoplasmic reticulum (ER) stress and mitochondrial apoptosis pathways owing to the altered regulation of caspase family members and ER stress-related molecules in MIN6 and primary islet cells.

    Article Title: Cell specific regulation of NaV1.7 activity and trafficking in rat nodose ganglia neurons
    Article Snippet: The supernatant was discarded, and the pellet was resuspended in complete DMEM media containing 1 % penicillin/streptomycin sulfate (10,000 μL −1 , stock), 30 ng mL −1 of nerve growth factor and 10 % fetal bovine serum (Hyclone). .. Collected nodose ganglion neurons were then transfected with Numb plasmid (pCMV-FLAG-Numb) , Invitrogen Stealth RNAiTM siRNA Negative Control (scramble siRNA) (cat. no. 12935300, Thermo Fisher Scientific) or CRMP2 siRNA (5′ GTAAACTCCTTCCTCGTGT-3′; obtained from Thermo Fisher Scientific) ( , , ) using the rat neuron Nucleofector TM solution (program O-003; Amaxa Biosystems, Lonza Cologne, Germany). .. The pmaxGFP TM Vector (program O-003; Amaxa Biosystems, Lonza Cologne, Germany) was transfected as a reporter gene.

    Synthesized:

    Article Title: The Role of MKP-5 in Adipocyte-Macrophage Interactions during Obesity
    Article Snippet: Human 293A cells from the ATCC were cultured using DMEM containing 10% FBS and penicillin/streptomycin. .. RNA Interference Small interfering RNA (siRNA) oligoribonucleotides targeting MKP-5 (siMKP5; Stealth RNAi TM siRNA, Cat. ‣8927232) and nonsense scramble siRNA (siSC; Stealth RNAi TM siRNA Negative Control, Cat. ‣12935300) were synthesized by Thermo Fisher Scientific. ..

    Article Title: The protective role of the MKP-5-JNK/P38 pathway in glucolipotoxicity-induced islet β-cell dysfunction and apoptosis.
    Article Snippet: Hyperglycemia and hyperlipidemia (glycolipotoxicity)-triggered islet β-cell dysfunction is known to drive the progression of obesity-related type 2 diabetes, however the underlying mechanisms have not been clearly elucidated.. The current study aimed to investigate the role of mitogen-activated protein kinase phosphatase 5 (MKP-5) in islet cells under glucolipotoxic conditions.. Using gene overexpression and knockdown approaches, we demonstrated that MKP-5 could alleviate glucolipotoxicity-induced apoptosis via the endoplasmic reticulum (ER) stress and mitochondrial apoptosis pathways owing to the altered regulation of caspase family members and ER stress-related molecules in MIN6 and primary islet cells.

    Transfection:

    Article Title: The protective role of the MKP-5-JNK/P38 pathway in glucolipotoxicity-induced islet β-cell dysfunction and apoptosis.
    Article Snippet: Hyperglycemia and hyperlipidemia (glycolipotoxicity)-triggered islet β-cell dysfunction is known to drive the progression of obesity-related type 2 diabetes, however the underlying mechanisms have not been clearly elucidated.. The current study aimed to investigate the role of mitogen-activated protein kinase phosphatase 5 (MKP-5) in islet cells under glucolipotoxic conditions.. Using gene overexpression and knockdown approaches, we demonstrated that MKP-5 could alleviate glucolipotoxicity-induced apoptosis via the endoplasmic reticulum (ER) stress and mitochondrial apoptosis pathways owing to the altered regulation of caspase family members and ER stress-related molecules in MIN6 and primary islet cells.

    Article Title: Insulin-independent stimulation of skeletal muscle glucose uptake by low-dose abscisic acid via AMPK activation
    Article Snippet: Transient transfection of L6 cells was performed using the Nucleofector System (Amaxa GmbH, Cologne, Germany). .. L6 (2 × 10 6 cells) were transfected using the Nucleofector program X-005 and R Kit with 2 μM rat LANCL2-targeting Stealth duplex short interference RNA (siRNA-L2, NM_001014187_stealth_208) or with 2 μM Stealth Negative Scramble Control (siRNA-SCR, NM_001014187_stealth_control_208) obtained from Invitrogen (Milan, Italy). ..

    Article Title: Cell specific regulation of NaV1.7 activity and trafficking in rat nodose ganglia neurons
    Article Snippet: The supernatant was discarded, and the pellet was resuspended in complete DMEM media containing 1 % penicillin/streptomycin sulfate (10,000 μL −1 , stock), 30 ng mL −1 of nerve growth factor and 10 % fetal bovine serum (Hyclone). .. Collected nodose ganglion neurons were then transfected with Numb plasmid (pCMV-FLAG-Numb) , Invitrogen Stealth RNAiTM siRNA Negative Control (scramble siRNA) (cat. no. 12935300, Thermo Fisher Scientific) or CRMP2 siRNA (5′ GTAAACTCCTTCCTCGTGT-3′; obtained from Thermo Fisher Scientific) ( , , ) using the rat neuron Nucleofector TM solution (program O-003; Amaxa Biosystems, Lonza Cologne, Germany). .. The pmaxGFP TM Vector (program O-003; Amaxa Biosystems, Lonza Cologne, Germany) was transfected as a reporter gene.

    Construct:

    Article Title: The protective role of the MKP-5-JNK/P38 pathway in glucolipotoxicity-induced islet β-cell dysfunction and apoptosis.
    Article Snippet: Hyperglycemia and hyperlipidemia (glycolipotoxicity)-triggered islet β-cell dysfunction is known to drive the progression of obesity-related type 2 diabetes, however the underlying mechanisms have not been clearly elucidated.. The current study aimed to investigate the role of mitogen-activated protein kinase phosphatase 5 (MKP-5) in islet cells under glucolipotoxic conditions.. Using gene overexpression and knockdown approaches, we demonstrated that MKP-5 could alleviate glucolipotoxicity-induced apoptosis via the endoplasmic reticulum (ER) stress and mitochondrial apoptosis pathways owing to the altered regulation of caspase family members and ER stress-related molecules in MIN6 and primary islet cells.

    Control:

    Article Title: Insulin-independent stimulation of skeletal muscle glucose uptake by low-dose abscisic acid via AMPK activation
    Article Snippet: Transient transfection of L6 cells was performed using the Nucleofector System (Amaxa GmbH, Cologne, Germany). .. L6 (2 × 10 6 cells) were transfected using the Nucleofector program X-005 and R Kit with 2 μM rat LANCL2-targeting Stealth duplex short interference RNA (siRNA-L2, NM_001014187_stealth_208) or with 2 μM Stealth Negative Scramble Control (siRNA-SCR, NM_001014187_stealth_control_208) obtained from Invitrogen (Milan, Italy). ..

    Plasmid Preparation:

    Article Title: Cell specific regulation of NaV1.7 activity and trafficking in rat nodose ganglia neurons
    Article Snippet: The supernatant was discarded, and the pellet was resuspended in complete DMEM media containing 1 % penicillin/streptomycin sulfate (10,000 μL −1 , stock), 30 ng mL −1 of nerve growth factor and 10 % fetal bovine serum (Hyclone). .. Collected nodose ganglion neurons were then transfected with Numb plasmid (pCMV-FLAG-Numb) , Invitrogen Stealth RNAiTM siRNA Negative Control (scramble siRNA) (cat. no. 12935300, Thermo Fisher Scientific) or CRMP2 siRNA (5′ GTAAACTCCTTCCTCGTGT-3′; obtained from Thermo Fisher Scientific) ( , , ) using the rat neuron Nucleofector TM solution (program O-003; Amaxa Biosystems, Lonza Cologne, Germany). .. The pmaxGFP TM Vector (program O-003; Amaxa Biosystems, Lonza Cologne, Germany) was transfected as a reporter gene.



    Similar Products

    90
    Thermo Fisher stealth sirna negative control (scramble)
    RT-qPCR validation of changes in expression of a subset of genes following BLID knockdown. RT-qPCR analysis of several genes in (A) MCF-7 and (B) MDA-MB-231 cells. β-Actin served as the internal control. (A) The y-axis title of the middle graph is identical to the y-axis title of the left graph. (B) The y-axis title of the middle-left graph is identical to the y-axis title of the far-left graph. *P<0.05 and **P<0.01 (BLID <t>shRNA</t> vs. the corresponding Scr shRNA group), n=3. shRNA, <t>short</t> <t>hairpin</t> <t>RNA;</t> <t>Scr,</t> <t>scramble</t> control; Ctl, control; RT-qPCR, reverse transcription-quantitative PCR; BLID, BH-3 like motif containing inducer of cell death.
    Stealth Sirna Negative Control (Scramble), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stealth+sirna+negative+control+(scramble)/stealth+rnai+sirna/pmc12217432-124-2-26
    Average 90 stars, based on 1 article reviews
    stealth sirna negative control (scramble) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher the scrambled negative control sirna (silencer select negative control or stealth negative control sirna)
    RT-qPCR validation of changes in expression of a subset of genes following BLID knockdown. RT-qPCR analysis of several genes in (A) MCF-7 and (B) MDA-MB-231 cells. β-Actin served as the internal control. (A) The y-axis title of the middle graph is identical to the y-axis title of the left graph. (B) The y-axis title of the middle-left graph is identical to the y-axis title of the far-left graph. *P<0.05 and **P<0.01 (BLID <t>shRNA</t> vs. the corresponding Scr shRNA group), n=3. shRNA, <t>short</t> <t>hairpin</t> <t>RNA;</t> <t>Scr,</t> <t>scramble</t> control; Ctl, control; RT-qPCR, reverse transcription-quantitative PCR; BLID, BH-3 like motif containing inducer of cell death.
    The Scrambled Negative Control Sirna (Silencer Select Negative Control Or Stealth Negative Control Sirna), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stealth+sirna+negative+control+(scramble)/pmc10805323-196-22-35
    Average 90 stars, based on 1 article reviews
    the scrambled negative control sirna (silencer select negative control or stealth negative control sirna) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher stealth rnaitm sirna negative control (scramble sirna)
    CRMP2 silencing does not affect sodium currents in rat nodose ganglion neurons. A. Western blot showing expression of CRMP2 in rat NGs (n = 4). β -III tubulin was used as a loading control. B. Representative family of Na + current traces from rat nodose ganglion neurons transfected with Scramble <t>siRNA</t> (control; black open circles) or CRMP2 siRNA (red open squares) and recorded 48 h after transfection. C. Summary of sodium current density versus voltage relationship. D. Bar graphs of total peak Na + current density from NGs transfected with the indicated siRNAs. No difference was observed between groups (Unpaired t test, n = 16 to 11 cells per condition). E. Boltzmann fits of normalized conductance ( G/Gmax ) voltage relationship for voltage dependent activation and inactivation of rat NGs transfected as indicated. No difference was observed in the activation or inactivation properties of both groups (Unpaired t test, n = 16 to 11 cells per condition; See Table S1 for detailed statistics). Error bars indicate mean ± SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Stealth Rnaitm Sirna Negative Control (Scramble Sirna), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stealth+sirna+negative+control+(scramble)/stealth+rnai+sirna/pmc09755031-80-13-23
    Average 90 stars, based on 1 article reviews
    stealth rnaitm sirna negative control (scramble sirna) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher stealth rnai sirna oligonucleotides negative control med gc scramble
    CRMP2 silencing does not affect sodium currents in rat nodose ganglion neurons. A. Western blot showing expression of CRMP2 in rat NGs (n = 4). β -III tubulin was used as a loading control. B. Representative family of Na + current traces from rat nodose ganglion neurons transfected with Scramble <t>siRNA</t> (control; black open circles) or CRMP2 siRNA (red open squares) and recorded 48 h after transfection. C. Summary of sodium current density versus voltage relationship. D. Bar graphs of total peak Na + current density from NGs transfected with the indicated siRNAs. No difference was observed between groups (Unpaired t test, n = 16 to 11 cells per condition). E. Boltzmann fits of normalized conductance ( G/Gmax ) voltage relationship for voltage dependent activation and inactivation of rat NGs transfected as indicated. No difference was observed in the activation or inactivation properties of both groups (Unpaired t test, n = 16 to 11 cells per condition; See Table S1 for detailed statistics). Error bars indicate mean ± SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Stealth Rnai Sirna Oligonucleotides Negative Control Med Gc Scramble, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stealth+sirna+negative+control+(scramble)/stealth+rnai+sirna+oligonucleotides+negative+control+med+gc+scramble/pm34485842-80-0-4
    Average 90 stars, based on 1 article reviews
    stealth rnai sirna oligonucleotides negative control med gc scramble - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher scramble sirna stealth rnaitm sirna negative control
    CRMP2 silencing does not affect sodium currents in rat nodose ganglion neurons. A. Western blot showing expression of CRMP2 in rat NGs (n = 4). β -III tubulin was used as a loading control. B. Representative family of Na + current traces from rat nodose ganglion neurons transfected with Scramble <t>siRNA</t> (control; black open circles) or CRMP2 siRNA (red open squares) and recorded 48 h after transfection. C. Summary of sodium current density versus voltage relationship. D. Bar graphs of total peak Na + current density from NGs transfected with the indicated siRNAs. No difference was observed between groups (Unpaired t test, n = 16 to 11 cells per condition). E. Boltzmann fits of normalized conductance ( G/Gmax ) voltage relationship for voltage dependent activation and inactivation of rat NGs transfected as indicated. No difference was observed in the activation or inactivation properties of both groups (Unpaired t test, n = 16 to 11 cells per condition; See Table S1 for detailed statistics). Error bars indicate mean ± SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Scramble Sirna Stealth Rnaitm Sirna Negative Control, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stealth+sirna+negative+control+(scramble)/scrambled+control+sirna+stealth+rnaitm+sirna+negative+control/pm31202710-73-19-32
    Average 90 stars, based on 1 article reviews
    scramble sirna stealth rnaitm sirna negative control - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher 2 µm stealth negative scramble control (sirna-scr, nm_001014187_stealth_control_208)
    CRMP2 silencing does not affect sodium currents in rat nodose ganglion neurons. A. Western blot showing expression of CRMP2 in rat NGs (n = 4). β -III tubulin was used as a loading control. B. Representative family of Na + current traces from rat nodose ganglion neurons transfected with Scramble <t>siRNA</t> (control; black open circles) or CRMP2 siRNA (red open squares) and recorded 48 h after transfection. C. Summary of sodium current density versus voltage relationship. D. Bar graphs of total peak Na + current density from NGs transfected with the indicated siRNAs. No difference was observed between groups (Unpaired t test, n = 16 to 11 cells per condition). E. Boltzmann fits of normalized conductance ( G/Gmax ) voltage relationship for voltage dependent activation and inactivation of rat NGs transfected as indicated. No difference was observed in the activation or inactivation properties of both groups (Unpaired t test, n = 16 to 11 cells per condition; See Table S1 for detailed statistics). Error bars indicate mean ± SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    2 µm Stealth Negative Scramble Control (Sirna Scr, Nm 001014187 Stealth Control 208), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stealth+sirna+negative+control+(scramble)/pmc06989460-195-32-40
    Average 90 stars, based on 1 article reviews
    2 µm stealth negative scramble control (sirna-scr, nm_001014187_stealth_control_208) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher scrambled stealth rnai™ sirna negative control kit (12935-100)
    CRMP2 silencing does not affect sodium currents in rat nodose ganglion neurons. A. Western blot showing expression of CRMP2 in rat NGs (n = 4). β -III tubulin was used as a loading control. B. Representative family of Na + current traces from rat nodose ganglion neurons transfected with Scramble <t>siRNA</t> (control; black open circles) or CRMP2 siRNA (red open squares) and recorded 48 h after transfection. C. Summary of sodium current density versus voltage relationship. D. Bar graphs of total peak Na + current density from NGs transfected with the indicated siRNAs. No difference was observed between groups (Unpaired t test, n = 16 to 11 cells per condition). E. Boltzmann fits of normalized conductance ( G/Gmax ) voltage relationship for voltage dependent activation and inactivation of rat NGs transfected as indicated. No difference was observed in the activation or inactivation properties of both groups (Unpaired t test, n = 16 to 11 cells per condition; See Table S1 for detailed statistics). Error bars indicate mean ± SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Scrambled Stealth Rnai™ Sirna Negative Control Kit (12935 100), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stealth+sirna+negative+control+(scramble)/10__1074_slash_jbc__ra119__008202-153-2-13
    Average 90 stars, based on 1 article reviews
    scrambled stealth rnai™ sirna negative control kit (12935-100) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher the scrambled stealth rnai tm sirna negative control kit (12935-100)
    CRMP2 silencing does not affect sodium currents in rat nodose ganglion neurons. A. Western blot showing expression of CRMP2 in rat NGs (n = 4). β -III tubulin was used as a loading control. B. Representative family of Na + current traces from rat nodose ganglion neurons transfected with Scramble <t>siRNA</t> (control; black open circles) or CRMP2 siRNA (red open squares) and recorded 48 h after transfection. C. Summary of sodium current density versus voltage relationship. D. Bar graphs of total peak Na + current density from NGs transfected with the indicated siRNAs. No difference was observed between groups (Unpaired t test, n = 16 to 11 cells per condition). E. Boltzmann fits of normalized conductance ( G/Gmax ) voltage relationship for voltage dependent activation and inactivation of rat NGs transfected as indicated. No difference was observed in the activation or inactivation properties of both groups (Unpaired t test, n = 16 to 11 cells per condition; See Table S1 for detailed statistics). Error bars indicate mean ± SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    The Scrambled Stealth Rnai Tm Sirna Negative Control Kit (12935 100), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stealth+sirna+negative+control+(scramble)/pmc06873174-449-2-14
    Average 90 stars, based on 1 article reviews
    the scrambled stealth rnai tm sirna negative control kit (12935-100) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher stealth rnai™ sirna negative control (scrambled sirna, 12935-200)
    CRMP2 silencing does not affect sodium currents in rat nodose ganglion neurons. A. Western blot showing expression of CRMP2 in rat NGs (n = 4). β -III tubulin was used as a loading control. B. Representative family of Na + current traces from rat nodose ganglion neurons transfected with Scramble <t>siRNA</t> (control; black open circles) or CRMP2 siRNA (red open squares) and recorded 48 h after transfection. C. Summary of sodium current density versus voltage relationship. D. Bar graphs of total peak Na + current density from NGs transfected with the indicated siRNAs. No difference was observed between groups (Unpaired t test, n = 16 to 11 cells per condition). E. Boltzmann fits of normalized conductance ( G/Gmax ) voltage relationship for voltage dependent activation and inactivation of rat NGs transfected as indicated. No difference was observed in the activation or inactivation properties of both groups (Unpaired t test, n = 16 to 11 cells per condition; See Table S1 for detailed statistics). Error bars indicate mean ± SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Stealth Rnai™ Sirna Negative Control (Scrambled Sirna, 12935 200), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stealth+sirna+negative+control+(scramble)/pm30528975-40-10-36
    Average 90 stars, based on 1 article reviews
    stealth rnai™ sirna negative control (scrambled sirna, 12935-200) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    RT-qPCR validation of changes in expression of a subset of genes following BLID knockdown. RT-qPCR analysis of several genes in (A) MCF-7 and (B) MDA-MB-231 cells. β-Actin served as the internal control. (A) The y-axis title of the middle graph is identical to the y-axis title of the left graph. (B) The y-axis title of the middle-left graph is identical to the y-axis title of the far-left graph. *P<0.05 and **P<0.01 (BLID shRNA vs. the corresponding Scr shRNA group), n=3. shRNA, short hairpin RNA; Scr, scramble control; Ctl, control; RT-qPCR, reverse transcription-quantitative PCR; BLID, BH-3 like motif containing inducer of cell death.

    Journal: Oncology Letters

    Article Title: BLID is a drug-responsive target of FOXO3a and multi-omics analysis reveals survival mechanisms and therapeutic vulnerabilities in BLID-deficient breast cancer cells

    doi: 10.3892/ol.2025.15155

    Figure Lengend Snippet: RT-qPCR validation of changes in expression of a subset of genes following BLID knockdown. RT-qPCR analysis of several genes in (A) MCF-7 and (B) MDA-MB-231 cells. β-Actin served as the internal control. (A) The y-axis title of the middle graph is identical to the y-axis title of the left graph. (B) The y-axis title of the middle-left graph is identical to the y-axis title of the far-left graph. *P<0.05 and **P<0.01 (BLID shRNA vs. the corresponding Scr shRNA group), n=3. shRNA, short hairpin RNA; Scr, scramble control; Ctl, control; RT-qPCR, reverse transcription-quantitative PCR; BLID, BH-3 like motif containing inducer of cell death.

    Article Snippet: In addition, Stealth siRNA negative control (scramble) with the same supplier's proprietary sequence designed to minimize sequence homology to any known vertebrate transcript (cat. no. 12935300, Thermo Fisher Scientific, Inc; Invitrogen) was used.

    Techniques: Quantitative RT-PCR, Biomarker Discovery, Expressing, Knockdown, Control, shRNA, Reverse Transcription, Real-time Polymerase Chain Reaction

    CRMP2 silencing does not affect sodium currents in rat nodose ganglion neurons. A. Western blot showing expression of CRMP2 in rat NGs (n = 4). β -III tubulin was used as a loading control. B. Representative family of Na + current traces from rat nodose ganglion neurons transfected with Scramble siRNA (control; black open circles) or CRMP2 siRNA (red open squares) and recorded 48 h after transfection. C. Summary of sodium current density versus voltage relationship. D. Bar graphs of total peak Na + current density from NGs transfected with the indicated siRNAs. No difference was observed between groups (Unpaired t test, n = 16 to 11 cells per condition). E. Boltzmann fits of normalized conductance ( G/Gmax ) voltage relationship for voltage dependent activation and inactivation of rat NGs transfected as indicated. No difference was observed in the activation or inactivation properties of both groups (Unpaired t test, n = 16 to 11 cells per condition; See Table S1 for detailed statistics). Error bars indicate mean ± SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: Neurobiology of Pain

    Article Title: Cell specific regulation of NaV1.7 activity and trafficking in rat nodose ganglia neurons

    doi: 10.1016/j.ynpai.2022.100109

    Figure Lengend Snippet: CRMP2 silencing does not affect sodium currents in rat nodose ganglion neurons. A. Western blot showing expression of CRMP2 in rat NGs (n = 4). β -III tubulin was used as a loading control. B. Representative family of Na + current traces from rat nodose ganglion neurons transfected with Scramble siRNA (control; black open circles) or CRMP2 siRNA (red open squares) and recorded 48 h after transfection. C. Summary of sodium current density versus voltage relationship. D. Bar graphs of total peak Na + current density from NGs transfected with the indicated siRNAs. No difference was observed between groups (Unpaired t test, n = 16 to 11 cells per condition). E. Boltzmann fits of normalized conductance ( G/Gmax ) voltage relationship for voltage dependent activation and inactivation of rat NGs transfected as indicated. No difference was observed in the activation or inactivation properties of both groups (Unpaired t test, n = 16 to 11 cells per condition; See Table S1 for detailed statistics). Error bars indicate mean ± SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Collected nodose ganglion neurons were then transfected with Numb plasmid (pCMV-FLAG-Numb) , Invitrogen Stealth RNAiTM siRNA Negative Control (scramble siRNA) (cat. no. 12935300, Thermo Fisher Scientific) or CRMP2 siRNA (5′ GTAAACTCCTTCCTCGTGT-3′; obtained from Thermo Fisher Scientific) ( , , ) using the rat neuron Nucleofector TM solution (program O-003; Amaxa Biosystems, Lonza Cologne, Germany).

    Techniques: Western Blot, Expressing, Control, Transfection, Activation Assay

    Gating properties of voltage-gated sodium channels in rat nodose ganglion neurons.

    Journal: Neurobiology of Pain

    Article Title: Cell specific regulation of NaV1.7 activity and trafficking in rat nodose ganglia neurons

    doi: 10.1016/j.ynpai.2022.100109

    Figure Lengend Snippet: Gating properties of voltage-gated sodium channels in rat nodose ganglion neurons.

    Article Snippet: Collected nodose ganglion neurons were then transfected with Numb plasmid (pCMV-FLAG-Numb) , Invitrogen Stealth RNAiTM siRNA Negative Control (scramble siRNA) (cat. no. 12935300, Thermo Fisher Scientific) or CRMP2 siRNA (5′ GTAAACTCCTTCCTCGTGT-3′; obtained from Thermo Fisher Scientific) ( , , ) using the rat neuron Nucleofector TM solution (program O-003; Amaxa Biosystems, Lonza Cologne, Germany).

    Techniques: Activation Assay, Control